Journal: PLOS One

Article Title: TLR9/MyD88/NF-κB signaling mediates mental stress-induced exacerbation of psoriasis through immune dysregulation in a mouse model

doi: 10.1371/journal.pone.0344474

Figure Lengend Snippet: (A-F): qRT-PCR was used to detect the expression level of TLR9, MyD88, NF-κB p65, TGF-β, IL-10 and IL-17 mRNA; (G-I): The concentrations of TGF-β, IL-10 and IL-17 were detected by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The samples were then incubated with a primary antibody targeting p-NF-κB p65 (Proteintech), IL-1β (Proteintech), IL-6 (Abcam), IL-17 (Proteintech) and IL-23 (Abcam) (all at a dilution of 1:50).

Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: PLOS One

Article Title: TLR9/MyD88/NF-κB signaling mediates mental stress-induced exacerbation of psoriasis through immune dysregulation in a mouse model

doi: 10.1371/journal.pone.0344474

Figure Lengend Snippet: (A) : The images of the affected skin in the different groups were photographed. (B) : Sucrose preference rate and stationary time were assessed by sucrose preference test and tail suspension test respectively. (C) : HE analysis of skin tissue in each group, scale bar = 50 μm; (D-F) : The expression levels of TLR9, MyD88, and NF-κB p65 in PBMCs of mice were detected by qRT-PCR; (G) The fluorescence intensity of p-NF-κB p65 was assessed by immunofluorescence test, scale bar = 100 μm; (H) The expression levels of TGF-β, IFN-γ, IL-1β, IL-6, IL-17 and IL-23 in PBMCs of mice were detected by qRT-PCR; (I) The fluorescence intensities of IL-1β, IL-6, IL-17 and IL-23 were assessed by immunofluorescence test, scale bar = 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The samples were then incubated with a primary antibody targeting p-NF-κB p65 (Proteintech), IL-1β (Proteintech), IL-6 (Abcam), IL-17 (Proteintech) and IL-23 (Abcam) (all at a dilution of 1:50).

Techniques: Suspension, Expressing, Quantitative RT-PCR, Fluorescence, Immunofluorescence

Journal: PLOS One

Article Title: TLR9/MyD88/NF-κB signaling mediates mental stress-induced exacerbation of psoriasis through immune dysregulation in a mouse model

doi: 10.1371/journal.pone.0344474

Figure Lengend Snippet: Following treatment with the specific inhibitors of the TLR9/MyD88/NF-κB pathway, (A): The protein levels of TLR9, MyD88, p-NF-κB p65 and NF-κB p65 in PBMCs of mice were measured by western blotting; (B) The fluorescence intensity of p-NF-κB p65 was assessed by immunofluorescence test, scale bar = 100 μm; (C): The serum concentrations of TGF-β, IFN-γ, IL-1β, IL-6, IL-17 and IL-23 were detected by ELISA; (D) The fluorescence intensities of IL-1β, IL-6, IL-17 and IL-23 were assessed by immunofluorescence test, scale bar = 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The samples were then incubated with a primary antibody targeting p-NF-κB p65 (Proteintech), IL-1β (Proteintech), IL-6 (Abcam), IL-17 (Proteintech) and IL-23 (Abcam) (all at a dilution of 1:50).

Techniques: Western Blot, Fluorescence, Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: PLOS One

Article Title: TLR9/MyD88/NF-κB signaling mediates mental stress-induced exacerbation of psoriasis through immune dysregulation in a mouse model

doi: 10.1371/journal.pone.0344474

Figure Lengend Snippet: Following injection of LV-NC, LV-shTLR9, or LV-MyD88 in mice, (A) : Daily scores based on PASI. (B) : HE analysis of skin tissue in each group, scale bar = 50 μm; (C-E) : qRT-PCR was used to detect the expression level of TLR9, MyD88, and NF-κB p65 mRNA in PBMCs of mice; (F) The fluorescence intensity of p-NF-κB p65 was assessed by immunofluorescence test, scale bar = 100 μm; (G) : The concentrations of TGF-β, IFN-γ, IL-1β, IL-6, IL-17 and IL-23 were detected by ELISA; (H) The fluorescence intensities of IL-1β, IL-6, IL-17 and IL-23 were assessed by immunofluorescence test, scale bar = 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The samples were then incubated with a primary antibody targeting p-NF-κB p65 (Proteintech), IL-1β (Proteintech), IL-6 (Abcam), IL-17 (Proteintech) and IL-23 (Abcam) (all at a dilution of 1:50).

Techniques: Injection, Quantitative RT-PCR, Expressing, Fluorescence, Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Obstructive Sleep Apnea–Induced Hypertension Is Associated With Increased Gut and Neuroinflammation

doi: 10.1161/JAHA.122.029218

Figure Lengend Snippet: Plasma levels of interleukin‐17a ( A ) and interleukin‐10 ( B ). Systolic blood pressure ( C ) in sham and OSA rats treated with a nonimmune IgG or interleukin‐17a neutralizing antibody. n=5 in ( A and B ), n=5–6 in ( C ); * P <0.05 for Sham+IgG vs OSA+IgG, # P <0.05 of OSA+nIL‐17a vs OSA+IgG, *** P <0.001 using two‐way ANOVA ( A , B ) or 2‐way repeated measures ANOVA and multiple comparisons with Tukey's multiple comparisons test ( C ). Error bars represent ±SEM. BP indicates blood pressure; IgG, immunoglobulin G; and OSA, obstructive sleep apnea.

Article Snippet: Biotinylated anti‐rat interleukin‐17a (Rockland, No. 212‐406‐b32) was added and incubated for 2 hours at room temperature.

Techniques: Clinical Proteomics

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Obstructive Sleep Apnea–Induced Hypertension Is Associated With Increased Gut and Neuroinflammation

doi: 10.1161/JAHA.122.029218

Figure Lengend Snippet: Effects of interleukin‐17a neutralization on Tregs, T H 1, T H 17, TNFα+, and macrophages in the ileum ( A ), cecum ( B ), and brain ( C ) of sham and OSA rats. n=4 in ileum, n=6 in cecum and brain; * P <0.05, ** P <0.005, *** P <0.0005, **** P <0.0001 using 2‐way ANOVA with Tukey's multiple comparisons test. Error bars represent ±SEM. OSA indicates obstructive sleep apnea; T H , T helper cell; and Tregs, T regulatory cells.

Article Snippet: Biotinylated anti‐rat interleukin‐17a (Rockland, No. 212‐406‐b32) was added and incubated for 2 hours at room temperature.

Techniques: Neutralization

Journal: Frontiers in Endocrinology

Article Title: Ultrasound combined with microbubbles promotes diabetic wound healing by regulating macrophage polarization

doi: 10.3389/fendo.2026.1781906

Figure Lengend Snippet: Transcriptome sequencing results and validation. (A) Volcano plot of differentially expressed genes between the USMB group and the Model group; (B) GO enrichment analysis; (C) Principal component analysis (PCA) plot; (D) KEGG enrichment analysis of differentially expressed genes; (E) Gene set enrichment analysis (GSEA) and corresponding heatmap; (F) PCR validation of selected differentially expressed genes associated with the IL-17 signaling pathway based on sequencing results; (G, H) Validation of IL-17 signaling pathway: protein expression levels of IL-17B, TNF-α, IL-17β, and NF-κB, along with quantitative statistical results. Data are presented as Mean ± SD, n= 4; *p< 0.05, **p< 0.01.

Article Snippet: The membrane was blocked with 5% skim milk prepared in Tris-buffered saline with Tween (TBST), followed by overnight incubation with primary antibodies against IL-17B (1:800, BOSTER), NF-κB (1:2000, Proteintech), TNF-α (1:800, BOSTER), and IL-1β (1:1000, Proteintech).

Techniques: Sequencing, Biomarker Discovery, Expressing